Effect of different concentrations of green tea on Cryopreservation of German Shepherd dog

To investigate the Effect of adding different concentrations green tea extract as an antioxidant on specific physical semen characteristics German Shepherd dogs during various steps frozen processing this study was carried out. A total (of 20) good Ejaculate samples were collected in a plastic bag from aged 3-4 yearsIn locations (dog breeding).To achieve current study's main objectives, two experiments designed through periods extended beginning December 2021 until end May 2022. The investigations included studying properties raw Semen (ejaculate volume, color, consistency, motility percentage, progressive dead sperm abnormalities and hypo-osmotic swelling test (HOST). After that, diluted with Tris supplemented antioxidants. followed after cooling freezing (post-thawing). In experiment, ten ejaculates collected. Dilution performed T1, T2, T3) cooled slowly up to 5C equilibrated, packed into 0.5 ml straws, put vapor nitrogen (-196C) for 5-10 minutes stored 48and 72 hr liquid Nitrogen (-196C). Progressive (HOST) evaluated post-thawing (Submerging straws water bath at 37C 30 seconds). It can be concluded present that extender improves ability freeze, especially treatment T3 (0.01 mg/5 ml) T2 (0.05 ml). This additive decrease negative compared control group.